Interaction mechanism of neuropeptides and VGSCs
Clemens-Schöpf Institute of Organic Chemistry and Biochemistry
Neurotoxic peptides possess an enormous research potential as they are highly affine and selective against diverse therapeutically-relevant targets. Among these targets, voltage-gated sodium channels are of particular interest due to their role in generation and propagation of electrical impulses in the cells of human organism. Although the knowledge how peptidic toxins modulate the voltage-gated sodium channels could significantly contribute in e.g. pain treatment, the mechanisms of action of these peptides have not been clearly understood to date. Therefore, the major objective of this project is to shed light on mechanism of interaction between neuropeptides and voltage-gated sodium channels. This project is very interdisciplinary and combines peptide synthesis (manual and automated), various purification and characterization techniques including NMR spectroscopy and computational binding studies.
- Solid phase peptide synthesis
- Liquid-phase synthesis (Organic chemistry)
- Analytical and preparative HPLC, Mass spectrometry (ESI and MALDI TOF)
- NMR and CD spectroscopy
- Computational structural biology
The applicant will work on solid phase peptide synthesis and folding of the cysteine-rich neuropeptide and further purification and structural analysis.
One IREP student(s) can work on this project.
Pre-requisites or requirements for the project
• chemistry lab experience
• organic chemistry
• analytical chemistry
Literature and preparation
• solid phase peptide synthesis
• cysteine-rich neuropeptides
A. A. Tietze, et. al, Angew. Chem. Int. Ed. 2012, 51, 4058-4061.
until the end of August 2019