Research projects are now available for highly motivated Bachelor and Master students with strong interests in protein engineering and synthetic biology. Topics of interests include, but are not limited to:
• Engineering optical protein sensors (e.g. with fluorescent and/or bioluminescent read-outs) against biotechnologically and biomedically relevant analytes.
• Engineering synthetic protease switches and thereof composed signalling circuits to sense and actuate cellular functions following different molecular and physical inputs (e.g. light).
• Re-engineering natural and artificially engineered protein nanopores equipping them with tailored properties and functions while dissecting the underlying design principles.
State-of-the-art cloning methods including library assembly; Fluorescence high-throughput genetic screening in microtitre plates and colony on-plate; High-resolution analysis by fluorescence microscopy combined with microfluidics; Recombinant protein expression, purification and characterisation;
 Gräwe A and Stein V. ‘Linker Engineering in the Context of Synthetic Protein Switches and Sensors‘. Trends in Biotechnology, 2021, 39, 731-744.
 Gräwe A, Ranglack J, Weyrich A and Stein V ‘iFLinkC: An iterative Functional Linker Cloning Strategy for the Combinatorial Assembly and Recombination of Linker Peptides with Functional Domains’. Nucleic Acids Research, 2020, 48, e24.
 Weber W, Roeder M, Abujubara H, Koeppl H, Tietze A and Stein V ‘The Functional Nanopore (FuN) Screen: A Versatile Genetic Assay to Study and Engineer Protein Nanopores in Escherichia coli’, 2021, bioRxiv, DOI: 10.1101/2021.04.20.440580.