This master thesis focuses on establishing the RNA detection in Saccharomyces cerevisiae using fluorescence in situ hybridization.
It will be a challenge to explore the localization and quantify the RNA during the data analyse and further compare it with in vivo data.
• Experience with cell biological working techniques, ideally experience in imaging
• Independent working ability
• Fluent English language skills in both reading and writing, German language skills beneficial
Contact for applications
Applications (including CV) should be sent in electronic form only, summarised in a single overall pdf document to email@example.com.
 Huber D, Voith von Voithenberg L, Kaigala G V. Fluorescence in situ hybridization (FISH): History, limitations and what to expect from micro-scale FISH Micro Nano Eng. 2018;1:15-24. doi:https://doi.org/10.1016/j.mne.2018.10.006
 Trcek T, Chao JA, Larson DR, Park HY, Zenklusen D, Shenoy SM, Singer RH. Single-mRNA counting using fluorescent in situ hybridization in budding yeast. Nat Protoc. 2012 Feb 2;7(2):408-19. doi: 10.1038/nprot.2011.451. PMID: 22301778; PMCID: PMC4112553.
 Sanderson MJ, Smith I, Parker I, Bootman MD. Fluorescence microscopy. Cold Spring Harb Protoc. 2014;2014(10):pdb.top071795-pdb.top071795. doi:10.1101/pdb.top071795