Who can apply?
Authors of theses related to synthetic biology are encouraged to apply. For each qualification level one award is available in each year:
BSc Thesis Award (250 €)
MSc Thesis Award (500 €)
PhD Thesis Award (1500 €)
Individuals who completed one of the respective degree programs at TU Darmstadt in the year 2022 are eligible. Candidates can submit their applications directly.
An application contains the electronic form of the thesis together with a brief executive summary highlighting its contribution to the field of synthetic biology (1 page) and a CV of the candidate. The selection committee consists of three founding members of the Centre for Synthetic Biology. Notifications will be sent out by 28. February 2023.
Please submit the application electronically in the PDF file format until 31. January 2023 to the managing director of the Centre Dr. Melanie Mikosch-Wersching (email@example.com).
Award winners 2021
In 2021, the following people were awarded the Centre Prize for their theses:
“In his bachelor thesis Ron Endruszeit tested the extend to which membrane proteins of the autotransporter family enable efficient surface display of complex fusion proteins in E. coli, and demonstrated the suitability of the system for protein-interaction screening by immobilization of binding partners on the cell surface. From the repertoire generated via the cloning strategy iFLinkC, a dependence of the protein-target interaction on the properties of the varied linkers could be concluded. In the future, the system should enable the fast and customized selection and isolation of protein variants from cell libraries.”
In her master's thesis, Sarah Schmitt worked on the recombinant modification of nanopores. With the help of protein engineering, nanopores can not only be better understood in terms of how they function, but their properties can also be modified, paving the way for biotechnological use. Furthermore, a native nanopore was modified to combine its own perforation properties with regulatory and sensory features.
In his dissertation, Dr Jan Bogen developed a variety of new methods for the generation, screening and engineering of tumour-specific antibodies. These included a novel method for the construction of immune libraries based on bidirectional vector systems, an approach for the rapid and effective humanisation of therapeutic drug candidates and for the generation of biparatopic antibodies. The molecules isolated in this work were adapted and optimised so that they could be combined into a trispecific, multifunctional antibody. This was able to prevent both the growth of cancer cells and their masking from the immune system, and at the same time mediated the attack of immune cells on the tumour cells. This molecule is thus unique in its mode of action and paves the way for new therapeutic approaches in the future.
Award winners 2020
In 2020, the following people were awarded the Centre Prize for their theses:
In her bachelor thesis, Theresa Wörmann constructed a fluorescent protein sensor against the biotechnologically relevant macrolide rapamycin and successfully applied it to quantitatively measure the flux of rapamycin across the cell membrane of E.coli in dependence on pore-forming membrane peptides. The bachelor thesis opens up a novel approach to systematically investigate the permeability of biotechnologically relevant products – an important but largely overlooked aspect in industrial biotechnology.
In his bachelor thesis, Christoph Reich developed a neural network – technically a generative adversarial network – to synthesize temporal multi-domain image sequences and showcased it with short time-lapse fluorescence microscopy imagery of yeast. These in silico simulations capture underlying biophysical factors and time dependencies, such as cell morphology, growth, physical interactions, as well as the intensity of a fluorescent reporter protein. The thesis is a first step towards completely in silico experimentation and in the future, this approach may complement and augment classical experimentation and mathematical modeling. The thesis is available on arXiv (https://arxiv.org/abs/2103.11834), along with a follow-up paper recently accepted to MICCAI 2021 (https://arxiv.org/abs/2106.08285).
In his doctoral thesis, Alexander Gräwe developed fundamental strategies for the construction of molecular sensory systems. This included novel approaches for the systematic construction of molecular switches, as well as, in collaboration with chemists and material scientists, a process for functionalising nanoporous films with customised receptors. The developed methods are to be used in medical diagnostics or in environmental monitoring.